Composite

Part:BBa_K1688001

Designed by: Biosurfers   Group: iGEM15_Uppsala   (2015-09-15)

Mono-rhamnolipid genes

The enzymes responsible for producing the mono-rhamnolipids (biosurfactants) are called RhlA and RhlB which are encoded by the genes rhlA and rhlB respectively. The protein encoded by these two genes make up the subunits of rhamnosyltransferase I. The rhlA gene (Ba_K1688002) coding for RhlA enzyme creates a 3-(hydroxyalkanoyloxy) alkanoic acid (HAA) fatty acid tail and the rhlB gene (BBa_K1688003) coding for the enzyme RhlB connects this tail to a rhamnose molecule making a mono-rhamnolipid. The biobrick (BBa_K1688001) is a composite part of BBa_K1688002 and BBa_K1688003.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 87
    Illegal BamHI site found at 647
    Illegal XhoI site found at 823
    Illegal XhoI site found at 2137
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1030
    Illegal NgoMIV site found at 1751
    Illegal NgoMIV site found at 1864
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 312
    Illegal BsaI site found at 1380
    Illegal BsaI.rc site found at 496


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Categories
Parameters
None